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991.
992.
玉米耐旱自交系在干旱条件下的mRNA差异表达 总被引:3,自引:3,他引:3
用16%PEG-6000对玉米耐旱自交系“81565”进行模拟干旱处理,用DD-PCR技术研究干旱与正常浇水对照的mRNA差异表达,发现3个干旱条件下差异表达的特异片段MD1、MD2和MD3。MD1和MD2在干旱条件下减量表达,MD3为干旱诱导表达。序列分析和同源性比对表明,MD1与玉米叶绿体基因组中编码参与RNA转录本Ⅱ型内含子剪切的成熟酶的matK基因有93%的相似性,MD2与极端耐旱的Sporobolus stapfianus的丝氨酸/苏氨酸2C型蛋白磷酸酶基因PP2C的相似性达99%,MD3与属天冬氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase基因有99%的相似性。根据各自序列相似同源基因的功能推测,MD1、MD2和MD3三个片段可能与“81565”的耐旱机制有关。 相似文献
993.
994.
滴施磷酸二氢钾后土壤中磷的移动和分布 总被引:2,自引:0,他引:2
在室内采用土柱模拟滴灌施P肥,研究了P在土壤中的移动和分布。试验结果表明:①滴施含P量200mg/L的KH2PO4后P在砂壤土、轻壤土、重壤土中分别纵向移动了22、16、14cm。横向和纵向移动的距离基本相同。②随着滴施P浓度的增加,P的移动距离增大,P的主要累积层加深。赤红壤中滴施P浓度200、400、800、1000mg/L时,P分别移动了12、16、20、22cm,而且有效P和全P含量增加显著的土层深度分别为0~8、0~12、0~18、0~20cm。在相同质地情况下,滴施高浓度P肥溶液可以明显增加P的移动距离。 相似文献
995.
Christophe Dambreville Catherine Hénault Florian Bizouard Thierry Morvan Rémi Chaussod Jean-Claude Germon 《Biology and Fertility of Soils》2006,42(6):490-500
The long-term (9 years) effect of pig slurry applications vs mineral fertilization on denitrifying activity, N2O production and soil organic carbon (C) (extractable C, microbial biomass C and total organic C) was compared at three soil depths of adjacent plots. The denitrifying activities were measured on undisturbed soil cores and on sieved soil samples with acetylene method to estimate denitrification rates under field or potential conditions. Pig slurry applications had a moderate impact on the C pools. Total organic C was increased by +6.5% and microbial biomass C by ≥25%. The potential denitrifying activity on soil suspension was stimulated (×1.8, P<0.05) 12 days after the last slurry application. This stimulation was still apparent, but not significant, 10 months later and, according to both methods of denitrifying activity measurement (r
2=0.916, P<0.01 on sieved soil; r
2=0.845, P<0.001 on soil cores), was associated with an increase in microbial biomass C above a threshold of about 105 mg kg−1. The effect of pig slurry on denitrification and N2O reduction rates was detected on the surface layer (0–20 cm) only. However, no pig slurry effect could be detected on soil cores at field conditions or after NO3
− enrichments at 20°C. Although the potential denitrifying activity in sieved soil samples was stimulated, the N2O production was lower (P<0.03) in the plot fertilized with pig slurry, indicating a lower N2O/(N2O + N2) ratio of the released gases. The pig-slurry-fertilized plot also showed a higher N2O reduction activity, which is coherent with the lower N2O production in anaerobiosis. 相似文献
996.
Oxidation of elemental sulfur (S0) in 20 Chinese agricultural soils was tested and the effects of previous S0 applications on the oxidation of additional applied S0 in selected soils were investigated using laboratory, greenhouse, and field experiments. Results showed that sulfur oxidative capacities presented great variability among 20 tested soils, with a coefficient of variation of 92.4%. There were no significant relationships between S0 oxidation and physical and chemical properties of the selected soil. Previous S0 amendment significantly increased the oxidation rate of additional applied S0. These stimulatory effects after the first applications of S0 were greater than those after two applications. The percent increase in S0 oxidation rate due to S0 pretreatment was negatively correlated with the oxidation capacities of soils before S0 pretreatments. The significant reduction of sulfur oxidation in autoclaved soils and significant increase in S0 oxidation after inoculation with S0-treated soil suspension demonstrated that microbial oxidation was mainly responsible for the enhancement of soil oxidation ability after previous S0 amendments. 相似文献
997.
Under semiarid subtropical field conditions, denitrification was measured from the arable soil layer of an irrigated wheat–maize cropping system fertilized with urea at 50 or 100 kg N ha–1 year–1 (U50 and U100, respectively), each applied in combination with 8 or 16 t ha–1 year–1 of farmyard manure (FYM) (F8 and F16, respectively). Denitrification was measured by acetylene inhibition/soil core incubation method, also taking into account the N2O entrapped in soil cores. Denitrification loss ranged from 3.7 to 5.7 kg N ha–1 during the growing season of wheat (150 days) and from 14.0 to 30.3 kg N ha–1 during the maize season (60 days). Most (up to 61%) of the loss occurred in a relatively short spell, after the presowing irrigation to maize, when the soil temperature was high and a considerable NO3–-N had accumulated during the preceding 4-month fallow; during this irrigation cycle, the lowest denitrification rate was observed in the treatment receiving highest N input (U100+F16), mainly because of the lowest soil respiration rate. Data on soil respiration and denitrification potential revealed that by increasing the mineral N application rate, the organic matter decomposition was accelerated during the wheat-growing season, leaving a lower amount of available C during the following maize season. Denitrification was affected by soil moisture and by soil temperature, the influence of which was either direct, or indirect by controlling the NO3– availability and aerobic soil respiration. Results indicated a substantial denitrification loss from the irrigated wheat–maize cropping system under semiarid subtropical conditions, signifying the need of appropriate fertilizer management practices to reduce this loss. 相似文献
998.
Runoff and sediment losses from rough and smooth soil surfaces in a laboratory experiment 总被引:5,自引:0,他引:5
Soil surface roughness may significantly impact runoff and erosion under rainfall. A common perception is that runoff and erosion are decreased as a function of roughness because of surface ponding and increased hydraulic roughness that reduces effective flow shear stress. The objective of this study was to measure the effects of initial surface roughness on runoff and erosion under controlled laboratory conditions. Initially, rough and smooth surfaces were exposed to five simulated rainfall applications at 5% and 20% slopes. In all cases, runoff was delayed for the case of the initially rough surface; however, this effect was temporary. Overall, no statistical differences in either total runoff or erosion were measured on the 20% slope. At 5% slope, runoff was less on the rough surface for the first rainfall application but greater on the final three, probably due to the formation of a depositional seal in that case. This resulted in an overall insignificant difference in runoff for the sum of the five rainfall applications. Erosion was greater on the rougher slope at 5% steepness, probably due to concentration of flow as it moved around the roughness elements on the rougher slope. These results indicate that commonly held perceptions of the impact of soil surface roughness on runoff and erosion may not be entirely correct in all cases. 相似文献
999.
Matthias C. Rillig Emily R. Lutgen Philip W. Ramsey John N. Klironomos James E. Gannon 《Pedobiologia》2005,49(3):43
Arbuscular mycorrhizal fungi (AMF) are key components of ecosystems through their influence on plant communities and ecosystem processes. A major source of information regarding the importance of AMF species richness on process rates are mesocosm experiments using different levels of diversity of AMF as provided by single-species cultures of AMF. Since AMF inocula are generally made available in the form of non-sterile pot culture material, it is possible that AMF symbiosis-associated microbiota are at least partially responsible for some effects hitherto directly attributed to the AMF mycelium. Here, we provide evidence that microbiota associated with single-species cultures of AMF (after long-term pot culture enrichment of 7–8 years) can strongly affect the ecosystem process of soil aggregation. This effect occurred in an AMF isolate specific manner, but in the absence of live and active AMF mycelium. We additionally documented large differences in microbiota communities associated with the different AMF inocula (using PLFA analyses), suggesting that these differences were at least partly responsible for the observed changes in soil aggregation. This result points to AMF–microbiota interactions as a largely unexplored mechanism underlying soil aggregation (and potentially other ecosystem processes). We suggest that a reinterpretation of previous experiments using greenhouse-derived AMF cultures may be necessary, and the need to consider AMF symbiosis-associated microbiota in mechanistic studies of AMF and mycorrhizae in general is emphasized. 相似文献
1000.